摘要
根据已报道的犬科狗的Gdf8基因cDNA编码序列设计一对引物,利用RT-PCR技术扩增银狐的Gdf8基因cDNA的编码序列,将PCR产物连接到质粒pMD18-T载体上,然后转化到JM109大肠杆菌中,得到阳性克隆,同时进行序列的测定和分析。测序结果与已报道的Gdf8基因cDNA编码序列的大小相同,为1128bp。该序列与狗的同源性为99.2%,仅有9个核苷酸差异,推断的氨基酸序列仅有3个差异。
According to cDNA sequence of Gdf8 gene of dog, a pair of primers was designed b y the PrimerSelect software. With the primers, RT-PCR was performed and the cDNA fragment of Gdf8 gene in the silver fox was obtained. The fragment was cloned i nto the pMD18-T vector using TA clone method. The identified positive clone was sequenced. The result showed that the sequence comprised the complete coding se quence of Gdf8 gene and its length was 1128bp. Compared with dogs, 99.2% nuc leotides were homologous, and there were nine point mutations, which caused thre e mutations in the predicted amino acid sequences.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2005年第4期29-31,共3页
Journal of Northeast Forestry University
基金
东北林业大学优秀青年教师创新项目资助。
