人为采收后长春花中长春碱含量的动态变化

Dynamic Variation of Vinblastine Content in Catharanthus roseus by Hand Harvest

采用超声提取和高效液相色谱法,对人为采收后的野生长春花中长春碱含量的动态变化进行了比较分析.分析结果表明:人为采收在干扰长春花生长发育的同时也影响到长春碱的合成与积累.采收后20 d与采收前相比,长春花各部位的生物量降低,长春花叶片和茎中的长春碱含量也有所降低.采收后20～40 d,长春花不同部位的生物量均呈上升趋势;采收后40～60 d,长春花各部位的生物量增加相对稳定.采收后40 d时,长春花根、叶片和茎中长春碱含量均显著高于采收前;采收后60 d时,长春花不同部位长春碱含量略低于采收前.人为采收后长春花单株长春碱总量也有所降低.长春碱的这种动态变化可能与其对外界干扰的拮抗作用有关.

The dynamic variation of vinblastine content in different parts of Cathar anthus roseus was measured by ultrasonic extraction and HPLC. The results show ed that hand harvest disturbed individual growth, and had influences on the synt hesis and accumulation of vinblastine. The biomass and vinblastine content of le aves and shoots decreased 20 days after harvest compared with pre-harvest. Bioma ss of different parts of Catharanthus roseus increased from 20 to 40 days af ter harvest, reaching to a stable level from 40 to 60 days after harvest. Vinbla stine contents of roots, leaves and shoots 40 days after harvest were significan tly higher than pre-harvest. Contents of the different parts 60 days after harve st were a little bit lower than pre-harvest. The total contents of vinblastine f or individual plant decreased after harvest. This kind of variation of vinblasti ne content in Catharanthus roseus was proposed to be caused by vinblastine a ntagonistic reaction to environmental disturbance.