胆汁酸对失代偿期肝硬化大鼠肠道细菌过度生长和细菌移位的影响

Effects of bile acid on intestinal bacterial overgrowth and the incidence of bacterial translocation in decompensated cirrhotic rats

目的观察胆汁酸对失代偿期肝硬化大鼠肠道细菌过度生长和移位的影响,为其临床应用提供依据。方法将37只伴有腹水的肝硬化大鼠及30只健康大鼠随机分为三组,分别采用结合胆汁酸甘氨胆酸(CG)、非结合胆汁酸熊去氧胆酸(UDCA)[均为70mg/(kg·d)]和安慰剂治疗2周。测定胆汁分泌速度、回肠细菌总数、细菌移位率。结果安慰剂组肝硬化大鼠胆汁分泌速度显著低于健康大鼠(P<0.001);胆汁酸治疗后速度增至正常水平。肝硬化大鼠回肠细菌总量显著高于健康大鼠(P<0.001),予胆汁酸治疗后降至正常水平。予胆汁酸的肝硬化大鼠细菌移位发生率显著低于安慰剂治疗组(P<0.01),存活率显著高于安慰剂组。结论失代偿期肝硬化大鼠应用结合或非结合胆汁酸可增加胆汁分泌、抑制肠道细菌过度生长,减少细菌移位的发生率,提高存活率。

Objective To evaluate the effects of bile acid on intestinal bacterial overgrowth and the incidence of bacterial translocation in decompensated cirrhotic rats.Methods 37 ascitic cirrhotic rats induced by CCl4 and 30 healthy rats were randomized to three groups which treated with cholyglycine、ursodeoxycholic acid (both at 70mg/kg/d),or placebo for 2 weeks respectively.At the end of treatment,the rate of bile secretion,total bacteria in terminal Ileum,bacterial translocation in ascitic cirrhotic rats and healty rats were measured.Results In cirrhotic rats reveiving placebo,bile secretion rate was significantly lower than that in healthy rats( P<0.001).The administration of bile acids to cirrhotic rats normalized bile secretion.Total ileal bacterial content was significantly higer in ascitic cirrhotic rats feeding placebo than that in healthy rats(P<0.001).Bile acid administraion reduced bacterial content to normal levels.Bacterial translocation was less in cirrhotic rats feeding bile acids than in rats receiving placebo(P<0.01).Survival rate was greater in rats receiving bile acids.Conclusions Administration of conjugated or unconjugated bile acids to decompensated cirrhotic rats resulted in an increase of bile acid secretion,an inhibition of intestinal bacterial overgrowth,a reduction of bacterial translocation,and a raise of survival.