基质影响电喷雾离子化效率的研究

Research on the matrix effect of electrospray ionization efficiency

着眼于液相色谱与质谱条件的匹配问题,以酸性化合物———大黄酸,碱性化合物———青藤碱为模型化合物,系统地考察了正离子和负离子模式下,6种常见的LC MS缓冲体系对电喷雾离子化效率的基质影响。结果表明:被分析物,采用不同的溶剂体系,离子化效率有显著差异,在实际样品分析中,应根据感兴趣的化合物选择合适的缓冲溶剂体系。

By investigating the MS behaviors of sinomenine (basic model compound) and rhein (acidic model compound) at five concentrations in six different kinds of buffer, we have investigated matrix effect in positive and negative ion mode to study the LC buffer systems matching MS analysis from system adaptability and signal intensity improvement. These results show different buffer systems have different matrix effects on ionization efficiency, and we should select appropriate buffer according to the analytes.