银杏叶提取物中黄酮类化合物的分光光度分析研究

Spectrophotometric determination of flavone in the extract of ginkgo biloba leaves (FEGb)

建立了一种银杏叶提取物中黄酮类化合物的分光光度测定方法。研究表明在NaNO2存在下,pH在13.20～13.60范围内,银杏叶提取物与Al(NO3)3形成稳定的粉红色配合物,于λmax=510nm处进行分光光度分析。标准品选用芦丁,其标准曲线在4.606～55.27μg/mL范围内服从朗伯-比尔定律,相关系数为r=0.9998。回收率为98.58%～102.80%,RSD为0.111%。可用于银杏叶提取物中黄酮类化合物的测定。

A Spectrophotometric method for the determination of Flavone in the extract of ginkgo biloba leaves (FEGb) was established. In the solution of NaNO_2 and pH 13.20～13.60, FEGb reacts with Al(NO_3)_3 to form a stable and sensitive pink complex, its maximum absorption wavelength is λ_(max)=510 nm. The absorbance (A) was linear with the concentration of FEGb in the range of 4.606～55.27 μg/mL, the method could be opplied to indirect determination of FEGb.