乙酰胆碱酯酶催化水解产物的电化学行为

Electrochemical behavior of a hydrolysis product of acetylthiocholine with acethylcholinesterase

报道了乙酰硫代胆碱水解产物硫代胆碱在玻碳电极上的电化学行为。以乙酰硫代胆碱作底物,在一定条件下乙酰胆碱酯酶催化底物水解,生成电活性物质硫代胆碱。利用循环伏安法和线性扫描伏安法研究了酶催化水解产物硫代胆碱在玻碳电极上的电化学行为。结果表明:在0.1mol/L的B-R缓冲溶液(pH7.0)中,硫代胆碱有一灵敏的氧化峰,峰电位EP=0.32V(vs.SCE);该体系属具有吸附性的不可逆过程。实验测得电子转移数为2,电极反应速率常数k=0.29s-1。

The electrochemical behavior of thiocholine at a glassy carbon electrode was presented. With acetylthiocholine (ATCh) as an substrate, an electroactive substance thiocholine was produced by the hydrolysis of ATCh catalyzed by acetylcholinesterase. The electrochemical behavior of thiocholine at the glassy carbon electrode was studied by cyclic voltammetry and linear sweep voltammetry. The results showed that a sensitive oxidation peak of thiocholine was observed in 0.1 mol/L B-R buffer solution (pH 7.0). The peak potential was 0.32 V (vs. SCE). The experiments have proved that the peak is an irreversible adsorption wave. The number of electron transfer is 2, and the electrode reaction rate constant k is 0.29 s^(-1).