野生黄刺玫的组织培养和快繁技术研究

Tissue Culture and Rapid Propagation Technique of wild Rose xanthina

以野生黄刺玫为外植体,探讨了不同生长调节物质对外植体分化、增殖和生根的影响,并对栽培基质与移栽技术进行研究。结果表明,利于黄刺玫分化的诱导培养基为MS+6-BA1.5mg.L-1+NAA0.1mg.L-1;以MS+6-BA1.0mg.L-1+NAA0.05mg.L-1作增殖培养基效果最好,增殖率为5.5;利于生根的培养基为1/2MS+IBA0.2mg.L-1;移栽基质为蛭石+珍珠岩+沙(1∶1∶1)效果最好,成活率97%,且苗生长健壮。

Using wild Rose xanthina of explants, we studied influencee of different growth regulating substances on explant differentiation, mutiplication and rooting, plant technique and plant materials. The results show that the best medium for induce was MS+6-BA 1.5 mg·L^(-1)+NAA 0.1 mg·L^(-1);that the best medium for the multiplication of medium was MS+6-BA 1.0 mg·L^(-1)+NAA0.05 mg·L^(-1), the multiplication rate was 5.5;the rooting and growing was the best in the medium with 1/2MS+IBA 0.2 mg·L^(-1);plant material was the best with the vermiculite +pearlite+sand(1∶1∶1), the sirvival rste was 97%.