淋球菌临床分离株青霉素耐药性与β-内酰胺酶基因相关性探讨

Correlation between penicillin resistance and beta–lactamase gene of Neisseria gonorrhoeae

目的了解淋球菌临床分离株青霉素耐药性与β-内酰胺酶基因存在的相关性.方法对2003年2～12月分离的128株淋球菌临床分离株用PCR法检测其特异的隐蔽质粒CPPB基因和青霉素耐药菌的β-内酰胺酶基因;用琼脂稀释法测定其对青霉素的最小抑菌浓度(MIC).结果 128株临床分离菌中CPPB基因阳性者125株,占97.7%.其中MIC≥1 mg/L的青霉素耐药菌86株,占CPPB基因阳性者的68.8%;MIC≥8 mg/L的高耐药菌59株,占耐药菌株总数的68.6%.86株耐药菌中β-内酰胺酶基因PCR扩增阳性者62株,占72.1%,其中58株(67.4%)为高耐药菌.结论研究表明,目前淋球菌临床分离株耐药情况较为严重,如果用青霉素治疗淋病需首先进行耐药检测.用PCR技术扩增CPPB基因有助于淋病的确诊.淋球菌的耐药性与β-内酰胺酶基因存在一定关系.β-内酰胺酶基因的检测对快速判断淋球菌是否高度耐受青霉素有临床指导意义.

Objective To investigate the penicillin resistance nature of Neisseria gonorrhoeae strains from clinical gonorrhea patients,and the relationship between antibiotic resistance and penicillinase gene existence.Methods 128 isolates of Neisseria gonorrhoeae during the period from January to December 2003 were examined.Results CPPB gene was tested positive by PCR in 125 from 128 strains(97.7%).Among the 125 strains,86 strains(68.8%)fell in MIC≥1 mg/ L; and 59 stains ( 68.6 %) fell in MIC≥8mg/ L.Penicillinase gene was tested positive by PCR in 62 strains from 86 penicillin resistance strains(72.1%),and that 58 strains(67.4%)from that MIC≥8 mg/L strains.Conclusion This study shows penicillin resistance is a big problem in clinical Neisseria gonorrhoeae ,and suggests certain relationship between Penicillin resistance and Penicillinase gene exsistence.Penicillinase gene testing by PCR is a hopeful method to predict the penicillin resistance.