东南沿海部队分离真菌菌株的药敏试验

An Antifungal Susceptibility Test in vitro of Isolates from Southeast Coastland Troops

目的对东南沿海部队真菌病调查中分离的皮肤癣菌,进行外用抗真菌药物敏感性的测定,进一步验证M38A方案。方法对致病真菌进行取样、培养、分离、纯化和分型,用微量稀释法测定药物的最小抑菌浓度。结果所选8种药物对受试真菌均有较好的敏感性,最小抑菌浓度分别为:咪康唑0.789μg/ml,益康唑0.246μg/ml,克霉唑0.136μg/ml,酮康唑1.043μg/ml,联苯苄唑0.461μg/ml,环吡酮胺0.412μg/ml,特比奈芬0.014μg/ml,布替奈芬0.020μg/ml。结论8种药物的MIC之间的差异有显著性,M38A有较好的重复性和稳定性,可作为一种较好的方法在实验室中应用。

OBJECTIVE A research on fungi was made in the southeast coastland troops. A M38-A scheme of (NCCLS) was used to evaluate the antifungal susceptibility of clinical isolates and to validate the M38-A scheme. METHODS After sampling, (culturing,) isolating, purifying and typing of fungi, to determine the minimum (inhibitory) concentration (MIC) with a microdilution method. RESULTS All the 8 kinds of drugs were sensitive to the isolates. Their MIC was: for miconazole (0.789 μg/ml,)econazole (0.246 μg/ml,)clotrimazole (0.136 μg/ml,)(ketoconazole) (1.043 μg/ml,) bifonazole (0.461 μg/ml,)ciclopirox (0.412 μg/ml,) terbinafine (0.014 μg/ml,)and butenafine (0.020 μg/ml. ) CONCLUSIONS There are significant differences among these 8 kinds of drugs. With good (reproducibility) and feasibility, the M38-A scheme can be used for the antifungal susceptibility test to dermatophytes in the laboratories.