高压电场低温萃取法制备生物可降解骨架的胰岛素缓释微球

Preparation of the controlled-released insulin microspheres by high-voltage and cryoextraction

目的:探索一种新型的胰岛素缓释微球制备方法。方法:采用高压电场低温萃取法,以生物可降解高分子材料聚乳酸乙醇酸共聚物(PLGA)为骨架材料,制备胰岛素缓释微球。结果:当药物与骨架材料的质量比为1:9时,可制得胰岛素载药微球其粒径<200μm,载药量为8.82%,包封率为83.2%,缓释达7d。结论:高压电场低温萃取法是一种工艺可行的新方法,为PLGA生物可降解型微球的制备提供了新方法。

Objective: To develop a new technique to prepare the controlled-released insulin microspheres. Methods: The insulin was mixed with a biodegradable poly-lactyl glycollic acid (PLGA) to form microscopic particles using a high-voltage electric field, followed by cryoextraction of the mixer to generate the microspheres. Results: At the ratio (w/w) 1:9 of insulin to PLGA, the microspheres with a diameter of less than 200μm reached the insulin loading rate of 8.82% and the complexing rate of 83.2 %. The insulin release from the microspheres was sustained for seven days. Conclusion: This high-voltage cryoextraction offers an innovative technique to prepare the controlled-released insulin microspheres.