调心方有效部位TX0201对Aβ25-35所致类“AD”大鼠氧化损伤及能量代谢的影响

Effects of HBR(调心方) TX0201 on the oxidative damage and the energy metabolism of AD rats causing by injection Aβ25-35 into amygdalas of both sides

目的研究调心方有效部位TX0201对杏仁核注射Aβ2535所致类AD模型大鼠氧化损伤及能量代谢的影响。方法通过杏仁核双侧注射Aβ2535片段造成类AD大鼠模型;采用分光光度法检测大脑皮层超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSHPX)活性、活性氧(ROS)的含量以及丙二醛(MDA)含量;原位杂交法检测皮层及海马细胞色素氧化酶Ⅱ型亚基(COⅡ)mRNA的表达。结果AD模型大鼠脑组织SOD、GSHPX活力明显下降,MDA及ROS含量显著上升,同时COIImRNA的表达也明显降低;治疗TX0201组和治疗E2020组大鼠脑组织SOD活力相对于模型组明显提高,MDA和ROS的含量则明显降低,GSHPX活力有升高的趋势;同时治疗TX0201组COIImRNA的表达较模型组有显著提高,而治疗E2020组COIImRNA的表达有上升趋势。结论调心方有效部位TX0201可以明显改善AD大鼠脑内氧化损伤以及能量代谢异常,防治AD的发生与发展。

To study the effect of TX0201 on Alzheimer's Disease rat model. We mainly observed the oxidative damage and the energy metabolism of the rats. Methods: We injected Aβ25-35 into amygdalas of both sides to make the 'AD' rat model. We detected the active of SOD?GSH-PX and the content of MDA?ROS in cortex by using spectropho meteric analysis;and in situ hybridization was used to detect the expression of COIImRNA in cortex and hippocampus. Results: Compared with the normal rats, the active of SOD?GSH-PX in the 'AD' rats' cortex was markedly decreased and the content of MDA?ROS in the 'AD' rats' cortex was obviously increased. Meanwhile,the expression of COIImRNA was evidently reduced. As for the rats in TX0201 group and E_2020 group comparing with the 'AD'rats, the active of SOD was obviously enhanced and the content of MDA?ROS was markedly decreased. However,the active of GSH-PX only had the trend of increasing. Meanwhile,comparing with the 'AD' rats, the expression of COIImRNA was obviously increased in the rats' brain of TX0201 group and in E_2020 group the expression only had the trend of increasing. Conclusion: We found that TX0201 had the effect of improving the oxidative damage condition and the abnormal energy metabolism in the 'AD' rats' brain.Thus,the recipe could prevent the occurrence and the development of AD.