PCR对儿童结核性脑膜炎的诊断价值

Significance of PCR in Diagnosis of Tuberculous Meningitis in Children

目的评价PCR对结核性脑膜炎(tuberculousmeningitisTBM)早期诊断的价值。方法采用PCR技术对20例TBM患儿进行脑脊液结核杆菌DNA检测并与抗PPD-IgG的检测结果相比较。结果两种诊断方法的敏感性和特异性分别为85%和89.3%,55%和85.7%,但两种诊断方法的阳性率与TBM的病程有关,在TBM的早期PCR检查的阳性率高,而抗PPD-IgG在TBM的晚期阳性率高。结论PCR检查是早期诊断TBM最敏感和特异的方法,如能与抗PPD-IgG同时检测将会提高TBM诊断的阳性率。

Objective To evaluate value of PCR useed for the early diagnosis of tuberculous meningitis (TBM). Methods 20 cases TBM children were studied by identification of genomic DNA of tuberculous in cerebrospinal fluid by PCR and comparing with the determination of anti-PPD-IgG. Results The sensitivity and specificity of the two tests were 85% and 89.3%, 55% and 85.7%. The positive rates of the two tests were correlated with the course of TBM.PCR positive rate is higher in early stage of TBM,while anti-PPP-IgG positive rate is high at late stage of TBM. Conclusion PCR would be more sensitive and specific for the early diagnosis of TBM and positive rate would be increased by the determination of anti-PPD IgG at the same time.