摘要
2mmol/L的SFE能显著地提高大豆蔗糖酶的活力。SDS-PAGE结果表明SFE主要通过增加大豆叶片蔗糖酶的生物合成量来大幅度地提高蔗糖酶的活力。用转录抑制剂AMD(8mg/L)和翻译抑制剂CHI(1.5mg/L)处理大豆叶片,发现它们都能在较短时间内大幅度地降低蔗糖酶的活性。SFE对大豆开花期和结荚期的蔗糖酶生物合成量的增加是通过SFE促进蔗糖酶的转录水平和翻译水平来实现的,转录起主要作用。
Results showed that 2mmol/L of sucrose fatty acid ester (SFE) could increase the activity of invertase in soybean leaves, but no effects on the permeability of cell membrane including the leakage of K + and reducing glucose. The analysis of scanning on SDS-PAGE gels showed that the increase of invertase activity after treated with 2mmol/L of SFE was caused by SFE enhancing the content of increase. Experiments also indicated that 8mg/L of actinomycin D (AMD, a transcription inhibitor) and 1.5mg/L of cycloheximede (CHI, a translation inhibitor) could decline the activity of invertase promoted by SFE in very short period obviously. It was suggested that SFE could increase invertase de novo biosynthesis on the level of the transcription mainly.
出处
《大豆科学》
CAS
CSCD
北大核心
2005年第2期95-100,105,共7页
Soybean Science
基金
西南师范大学自然基金项目资助
