6株生防细菌对大豆根腐病防治效果初步评价

EVALUATION OF SIX POTENTIAL BIOCONTROL AGENTS AGAINST SOYBEAN ROOT ROT

从大豆根际分离出的1500多株细菌中筛选出6株生防细菌,其在离体的条件下对大豆根腐病病原菌尖孢镰刀菌(Fusarium oxysporum )有很好的抑制作用,其中菌株18BRR2-2、18BRR9-1和3BRR16-2在离体的条件下也对茄腐镰刀菌(F. solani)、半裸镰刀菌(F. semitectum) 和立枯丝核菌(Rhizoctonia solani)有抑制作用,并且这6株生防细菌的发酵液可以抑制尖孢镰刀菌(F. oxysporum )的生长。通过温室盆栽和大田试验表明,这些生防菌对大豆根腐病有很好的防治效果,其中菌株18BRR2-2盆栽和大田的防效均最高,分别达到了45.9%和65.00%。而且这些生防菌也表现出对大豆明显的促生作用。认为18BRR2-2 号菌株有开发为防治大豆根腐病生防制剂的潜力。

Six strains were screened from 1500 strains, which were isolated from the soybean rhizosphere soil.In vitro, they exhibited the antagonism toward the plant pathogenic Fusarium oxysporum. The strains 18BRR2-2,18BRR9-1,3BRR16-2 among the isolates had the antagonism activity toward F. solani,F. semitectum and Rhizoctonia solani. And, the secondary-metabolite produced by these isolates had the inhibitional activity to F. oxysporum.These isolates significantly reduced the incidence of soybean root rot under controlled glasshouse conditions and the field from 31.97% to 45.90% and 20.00% to 65.00% respectively.And these strains may promote the growth of soybeans seedling. The strain, 18BRR2-2 has the potential to form production to control the soybean root rot.