小鼠囊胚及孵化胚胎细胞计数方法的探讨

Exploring of Counting Method of Mouse Blastocyst and Hatched Blastocyst

实验以ICR小鼠体外培养的囊胚及孵化胚胎为材料,比较两种方法对小鼠胚胎细胞计数的效果。方法1为采用0.5%的柠檬酸钠低渗溶液和姬姆萨染液对囊胚及孵化胚进行压片,且不同时期胚胎低渗和染色处理时间相同;方法2染色液为苏木精染液,且不同时期胚胎低渗和染色处理时间不同。结果表明,方法2小鼠囊胚及孵化胚胎细胞球轮廓清晰,细胞间隙明显可见,其观察率分别为94.7%和96.8%;而方法1胚胎细胞球间隙不明显,一些细胞轮廓模糊,其观察率分别为70.5%和69.6%。小鼠囊胚及孵化胚胎采用压片计数法,以方法2显著好于方法1(P<0.05)。

The experiment was blastocyst and hatch blastocyst of ICR mouse culture in vitro, and the effect of two methods to the cell counting of mouse embryo was compared. Method 1 was that blastocyst and hatch blastocyst was pressed with the 0.5% low osmosis liquid of Sodium Citrate and Giemsa dye, and the treat time of low osmosis and dye of corresponding embryo was identical in different period;Method 2 was blastocyst and hatched blastocyst was pressed with Hematoxylin, and the treat time was different in different period.The results indicated that in method 2 the outline of mouse blastocyst and hatched blastocyst cell ball was clear, the cleft of cell was obvious, its rate of observation was 94.7% and 96.8% respectively; while in method 1 the cleft of embryo cell ball was unclear, and the outline of some cells was blurry, and its rate was 70.5% and 69.6% respectively.Method 2 is significantly better than method 1 by adopting count method of press of small mouse blastocyst and hatched blastocyst ( P<0.05) .