流式细胞技术在胃镜活检组织诊断中的应用价值

APPLICATION OF DNA FLOW CYTOMETRY IN THE HISTOLOGICAL DIAGNOSIS OF GASTRIC ENDOSCOPIC BIOPSIES

应用流式细胞仪（ＦＣＭ）对６２例临床常规胃镜活检组织进行检测，建立了常规胃锐活检组织的ＦＣＭ检测诊断标准，分为ＦＣＭ检测阳性（＋）、可疑（±）及正常（－）三级。慢性萎缩性胃炎及异型增生组中ＦＣＭ阳性率为２２％（４／１８），可疑为３３％（６／１８）．胃癌组中ＦＣＭ阳性检出率为７０％（１４／２０），２４例基本正常胃粘膜（包括浅表性胃炎）组均为二倍体ＤＮＡ。我们认为，ＦＣＭ可疑及阳性可能为重要的癌变标志，ＦＣＭ检测可用于胃癌前病变的动态观察。

Sixty-two samples from human gastric endoscopic biopsies were analysed in order to detect DNA ploidy content alterations as markers for cancerous and precancerous lesions of the stomach.The criterion for diagnosis by FCM consisted of FCM positive(+).suspicious(±)and negative(-).All the samples of normal mucosa(including superficial gastritis)showed a diploid DNA content.In the group of chronic atrophic gastritis and gastric dysplasia,positive rate was 22%(4/18) and suspicious rate was 33%(6/18).Whereas FCM positive rate was 70%in 20 patients with gastric cancer. It is likely that FCM positive and suspicious may be one of the important markers for cancerous and precancerous diagnosis.