酶免疫──醋酸纤维薄膜电泳法诊断大肠癌

THE CLINICAL USEFULNESS OF TUMOR ASSOCIATED ANTIGENS DETECTION IN PATIENTS WITH COLORECTAL CANCER BY ELECTROPHORESIS AND ENZYME STAINING

以抗结肠癌及胰腺癌的单克隆抗体用酶免疫──醋酸纤维薄膜电泳法，检测大肠癌及非癌疾病患者粪便中的癌相关抗原。其中大肠癌６１例，慢性肠炎２０例，正常人１００例。结果表明，四株单克隆抗体ＣＬ－３、ＣＬ－４、ＰＳ－２、ＰＳ－９对应抗原在大肠癌患者粪便中的平均含量显著高于正常对照组及非癌肠道疾病组，其阳性率分别为７５．４％、５９．４％，７２．５％和５１．３％，如将三株单抗ＣＬ－３、ＣＬ－４、ＰＳ－２组合应用，则大肠癌组阳性率达８６．２％，正常组为１５．２％，肠炎组为１６．６％，６５例大肠癌中，５例早期诊断（ＤｕｋｅｓＡ期）患者均为阳性。本方法可能为早期诊断大肠癌提供简便省时，价廉，便于推广的新方法。

By monoclonal antibodies tumor-associated antigens were assayed by electrophoresis and immuno-enzyme staining methods in fecal extracts from patients with colorectal cancer and non-malignant diseases. The results indicated that the average content of tumor-associated antigens in colorectal cancer patients are significantly higer than those in normal individuals and in non-malignant diseases. The McAbs positive rates in colorectal cancer are:CL-3,74.5%;CL-459.5%;PS-2 72.5%and PS-9 51.3%, When detected by cocktail of those three McAbs CL-3, CL-4 and PS-2, the positive rate of colorectal cancer is 86.2%normal individuals is 15.2%and enteritis is 16.6%. Five cases of Dukes A stage are all positive. This method may be useful for early diagnosis and mass screening of colorectal cancer,and it is easy to handle,time saving,money saving,and painless for patients.