人胎盘GST－π抗体的免疫组织化学检测对胆囊癌早期诊断的意义

Value of Immunhistochemical Investigatinn of Anti-GST-π Antibody in the Early Diagsis of the Gallbladder Carcinom

应用人胎盘型谷胱甘肽Ｓ—转移酶（ＧＳＴ－π）抗体的ＡＢＣ法免疫组织化学技术，检测了１００例胆囊良、恶性病变组织的ＧＳＴ－π活性，结果表明，２２例正常胆囊粘膜对照组ＧＳＴ－π阳性率为９％，１２例胆囊炎和４例胆囊腺瘤ＧＳＴ－π阳性率分别为１６．６７％和２５％，视为癌前病变的异型增生组，ＧＳＴ－π阳性率达１００％，随着异型增生程度加重，ＧＳＴ－π活性增强。３８例胆囊腺癌ＧＳＴ－π阳性率为８７％，其中高分化和中分化型腺癌组织中ＧＳＴ－π阳性率为９５％，低分化型为１００％，未分化型腺癌ＧＳＴ－π阳性率７５％，胆囊腺癌、异型增生组ＧＳＴ－π阳性率及平均强度都显著高于良性病变和正常对照组（Ｐ＜Ｏ．０１）。因此，ＧＳＴ－π的免疫组织化学检测可作为胆囊癌及癌前病变的一项新的早期诊断指标。

Human placental form of giutathione S-transferase (GST-π) was detected in38 gallbladder carcinoma and 24 atypical hyperplasis by Avindin biotin peroxidase complex (ABC) method using anti-GST-π antibody. of 22 normal gallbladder mucosa, 91% were negating for GST-π atain, 95% of 21 well and moderately differentialed gallbladder adenocarcinomas,all of poorly differentiated gallbladder adenocarcinomas, 75% of undifferentiated gallbladder Carcinomas, 100% of atypical hyperplasis were positive for GST-π stain. These results indicate that GST-π is a new sensitive marker for immunohistochmical detection of gallbladder carcinoma.