肠内营养制剂对短肠综合征大鼠血清游离脂肪酸代谢的影响

Effect of Enteral Nutrition Support on Serum Free Fatty Acids in Short-bowel Syndrome Rats

目的:观察短肠综合征(SBS)大鼠血清游离脂肪酸水平的变化,评价长链甘油三酯(LCT)和长链甘油三酯加中链甘油三酯(MCT/LCT,1∶1)肠内营养制剂对大鼠血清游离脂肪酸水平的影响。方法:SD大鼠随机分为3组(每组7只):假手术对照组,进行小肠离断再吻合并给予LCT营养支持;85%肠切除组,进行LCT营养支持;85%肠切除组,MCT/LCT营养支持。14d后取血清用气相色谱法测定大鼠血清游离脂肪酸水平。结果:SBS大鼠血清中总游离脂肪酸水平以及各游离脂肪酸水平明显低于对照组,大多差别具有显著意义。添加MCT的肠内营养大鼠其血清游离脂肪酸水平明显高于仅给予LCT脂肪乳剂的大鼠。结论:添加50%MCT的MCT/LCT肠内营养制剂较单纯LCT肠内营养制剂能增加SBS大鼠对脂肪的吸收功能,增加血清游离脂肪酸水平,而不影响肠道代偿能力。

AIM:To study the effect of enteral nutrition containing long chain triglycerides (LCT) and LCT containing 50% medium chain triglycerides (MCT/LCT) on serum free fatty acid (FFA) profiles in short bowel syndrome (SBS) rats.METHODS:Sprague-Dawley rats were randomly divided into 3 group (n=7):pseudo-operation fed with LCT as control;85% small bowel resection fed with LCT and with MCT/LCT,respectively.Serum FFA profiles were determined by gas chromatography after 14 days.RESULTS:Both total and most of individual serum FFA levels were significantly lower in rats with SBS compared with control group.After being treated with MCT,serum FFA levels in rats with SBS were significantly higher than those of LCT.CONCLUSION:SBS rats fed with enteral nutrition alone show a deficiency of serum FFA.Enteral nutrition containing MCT obviously increases serum FFA level and does not inhibit intestinal adaptation.