培养状态下成人骨髓基质细胞的生长特性

Growth characteristics of cultured adult bone marrow stromal cells in vitro

目的研究培养状态下成人骨髓基质细胞的生长特性,为自体骨组织工程的功能细胞培养提供实验基础研究。方法培养健康成人骨髓基质细胞连续8代,测定第3、6代细胞的生长曲线、分裂指数、贴壁率及倍增时间。结果所测第3、6代细胞生长曲线基本相同,12h内细胞贴壁率达88.54%,分裂指数最高达15‰。结论在本实验条件下,细胞生长较稳定,接种成活率较高,增殖速度较快,可用于自体骨组织工程。

Objective To investigate the growth characteristics of cultured adult bone marrow stromal cells (MSC) in vitro to provide the experimental basis of MSC used as the functional cells in bone tissue engineering. Methods Bone marrow was obtained by aspirated in the posterior superior iliac spine or flushed medullary cavity in costal bone from an adult patient whose marrow was normal. Mononuclear cells were then isolated by centrifugation for primary culture in different culture fluid systems. Subculture was done for 8 passages consecutively. The following data were obtained: growth curve, division rate, adhesive rate and double time. Results The growth curves of the third and sixth passages were very similar. The highest division rate of the third passage was 15‰. 88.54% of the subcultured cells were adhesive in 12 h. Conclusion MSC showed stable growth, relatively rapid proliferation and easy survival in the subculture in present culture condition. MSC in adults may be used in autogeneous bone engineering.