高效液相色谱法测定生血合剂中黄芩苷的含量

Determination of baicalin in Shengxue Heji by HPLC

目的建立测定生血合剂中黄芩苷的HPLC测定方法。方法黄芩苷测定以kromasilODSC18(4.6mm×200mm,5μm为固定相,甲醇水磷酸(53∶47∶0.2,V/V/V)为流动相,UV检测波长为280nm。结果黄芩苷在0.20～1.80μg范围内与峰面积呈直线关系。回归方程为:Y=251481.75X-6859.95,r=0.9999,平均回收率为99.66%,RSD=1.14%。结论本法测定生血合剂中黄芩苷的含量简便、准确、快捷,可用于生血合剂的质量控制。

OBJECTIVE To establish HPLC method for determination of baicalin in Shengxue Heji.METHODS Baicalin was separated on kromasil ODS C_ 18 (4.6mm×200mm,5μm)column and detected at 280nm, with methanol-water-phosphoric acid(53∶47∶0.2,V/V/V)as mobile phase. RESULTS There was good linearity from 0.20 to 1.80μg (Y=251481.75X-6859.95, r =0.9999) in separation of baicalin. The average recovery was 99.66% with RSD of 1.14%. CONCLUSION This method is simple, rapid, and accurate. This study provides the reference to establish quality standard for Shengxue Heji.