高效液相色谱法测定强阳灵口服液中淫羊藿苷的含量

Determination of icariin in Qiangyangling oral liquids by HPLC

目的建立高效液相色谱法测定强阳灵口服液中淫羊藿苷含量的方法。方法采用HPLC法,在波长284mm以乙腈水(23∶77)为流动相,测定淫羊藿苷的含量。结果淫羊藿苷在0.241～1.203μg范围内呈良好线性关系(r=0.9995)平均回收率为99.0%(RSD=1.0%,n=5)。测定5批样品的RSD为0.5～0.9%。结论所建立的方法可准确进行定量检测,可用于该制剂的质量控制。

OBJECTIVE To develop a HPLC method for determination of icariin Qiangyangling oral liquids.METHODS The method was performed on VP-BDS column,acetonitrile-water(23∶77)was used as mobile phase,the detection wavelength was 284nm.RESULTS The assay was linear over the range of 0.24 1~1.203μg, r =0.9995.The average recovery was 99.0%,with the RSD=1.0%.CONCLUSION The method developed is accurate,sensitive and repeatable.It can be applied to the quality control of Qiangyangling oral liquids.