白芍与柴胡不同比例配伍芍药苷水煎出量的比较

Influence of compatibility ratio of Radix Paeoniae Alba and Radix Bupleuri on the dissolution of paeoniflorin in water extract

目的考察白芍与柴胡不同比例配伍共煎芍药苷的煎出量,以探讨传统“药对”白芍配伍柴胡对有效成分煎出的影响。方法单味白芍、白芍与柴胡(1+2,1+1,2+1)配伍,水煎提取,分离,采用HPLC法,以ODSC18(4.6mm×250mm)为色谱柱,乙腈水=17∶83为流动相,流速:1.0mL/min,检测波长:230mm,柱温:室温。结果白芍配伍柴胡,芍药苷煎出量均比白芍单煎高,平均提高12.68%,其中白芍与柴胡2∶1配伍比例芍药苷煎出量最大。结论说明白芍配伍柴胡有利于芍药苷煎出,从而使芍药苷煎出量增加。

OBJECTIVE To investigate the influence upon the extracting efficiency of traditional“drug couples” Radix Paeoniae Alba concomitant with Radix Bupleuri by determining the content of paeoniflorin in the water extracts by boiling before and after combination.METHODS Single Radix Paeoniae Alba, Radix Paeoniae Alba concomitant with Radix Bupleuri (1+1,1+2,2+1) were boiled with water and separated. HPLC was used to determine the content of paeoniflorin. Column:ODS-C_ 18 (4.6mm×250mm). Mobilephase:acetonirile: water=17∶83. Flow rate:1.0mL/min. Detector:UV at 230nm. RESULTS The content of paeoniflorin was increased in Radix Peaoniae Alba water extracts with Radix Bupleuri , the average increased was 12.68%, the best of them was Radix paeoniae Alba extracts with Radix Bupleuri (1∶2). CONCLUSION Radix Paeonia Alba extracts with Radix Bupleuri, the contents of paeoniflorin by boiling was increased evidently.