Aroclor 1254对小鼠精子受精能力与受精卵发育的毒性研究

Toxicity of the aroclor 1254 on the fertilization ability of sperm and the development of early embryo in mice

采用腹腔注射染毒法研究了Aroclor1254对公鼠精子受精能力及受精卵发育的毒性。将体重(18±3)g的性成熟期公鼠随机分为1/10LD50,1/50LD50,1/250LD50和1/1250LD504个染毒组及稀释剂对照组,染毒组公鼠于试验的第0,3,6天,按照0.01mL/g的注射量,分别注射用灭菌花生油稀释的20,4,0.8,0.16mg/mLAroclor1254溶液,稀释剂对照组注射灭菌花生油。试验开始40d后,用各组公鼠对未染毒的超排母鼠配种。结果表明,用高剂量Aroclor1254染毒公鼠对超排的正常母鼠进行配种时,会显著降低受精卵比例(P<0.01)和受精卵的卵裂率(P<0.05),并出现受精卵首次卵裂延迟。在受精卵的进一步发育中,随着公鼠染毒剂量的增加,发育到桑椹胚和囊胚的比例显著降低,发育延迟胚及发育中退化变性胚比率显著升高。据此认为,Aroclor1254能明显影响小鼠精子受精能力,并影响受精卵的进一步发育。

The toxicity of the Aroclor 1254 on the fertilization ability of sperm and the development of early embryo in mice were studied.The puberty male mice with body weight (18±3) g were divided into 1/10 LD 50 ,1/50 LD 50 ,1/250 LD 50 ,1/250 LD 50 ,4 Aroclor 1254 dose groups and dilution control group randomly.The male mice were injected with abdominal cavity (0.01 mL/g) with 20,4,0.8,0.16 mg/mL Aroclor 1254 solution and peanut oil sterilized respectively.In the 0,3,6th days.After 40 days of experiment bigin,the male mice mated with untreated female mice.The result showed that the fertilizing rate was decreased and the first cleavage rate of the zygote was relayed in the 1/10 LD50 dose males.With the increase of the 1254 dosages,in the further development process of 2 cell embryo,there still occurred the development relayed and degeneration,so that the development rate of morulae and blastocysts decreased significantly.The results suggested that Aroclor 1254 could remarkably affect the fertilization ability of sperm and the development ability of embryo.