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安氟醚、异氟醚和七氟醚对大鼠脑缺氧缺糖损伤的保护作用 被引量:2

Protective Effect of Enflurane, Isoflurane and Sevoflurane on Brain Ischemia in Rat
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摘要 目的探讨安氟醚、异氟醚和七氟醚对大鼠皮层脑片缺氧缺糖损伤的保护作用及其机制。方法大鼠皮层脑片随机分为四组:对照组、损伤组(缺氧缺糖损伤10min)、吸入麻醉药+损伤组、印防己毒碱(GABAA受体拮抗剂)+吸入麻醉药+损伤组。通过脑片病理切片、HE染色和TTC染色、定量比色测定吸光度A值,观察脑片缺氧缺糖损伤程度。结果1.0MAC安氟醚、异氟醚和七氟醚处理30min后,皮层脑片神经细胞的病理损伤较损伤组明显减轻。1.0MAC和2.0MAC的安氟醚、异氟醚和七氟醚明显提高损伤所致脑片的低A值(P<0.01)。50μmol/L的印防己毒碱完全抑制了2.0MAC安氟醚的作用(P<0.01),部分抑制了2.0MAC异氟醚的作用(P<0.01),但不影响2.0MAC七氟醚的效果(P>0.05)。结论1.0MAC和2.0MAC的安氟醚、异氟醚和七氟醚对大鼠皮层脑片缺氧缺糖损伤均有一定的保护作用;GABAA受体参与了安氟醚和异氟醚的脑缺血保护作用. Objective To investigate the protective effects and its mechanisms of enflurane, isoflurane and sevoflurane on rat brain ischemia induced by oxygen-glucose deprivition (OGD) on rat cerebral cortical slices. Methods Slices of the rat cerebral cortex were made and divided into four groups: control group; injury group; volatile anesthetics+OGD injury group; picrotoxin+volatile anesthetics+OGD injury group. The histologic changes of brain slices were assessed by hematoxylin and eosin staining, the degree of injury and the effects of volatile anesthe- tics were quantitatively evaluated by A value of the absorbance at 490 nm of slices, which were co-incubated with TTC for 30 min. Results Compared to the slices of OGD injury groups, the histologic changes of the degenerated neurons (characterized by cytoplasmic swelling and pyknotic nuclei) were ameliorated in the slices preconditioned by volatile anesthetics. Compared to the control groups, OGD injury for 10 min decreased the A value of slices (P< 0.01). 1.0 and 2.0 MAC of enflurane, isoflurane and sevoflurane inhibited the decrease of A values induced by OGD injury (P<0.01). 50 μmol/L of picrotoxin abolished the increase of A value of slices preconditioned with 2.0 MAC of enflurane, partially decreased the increase A value of slices preconditioned with 2.0 MAC of isoflurane, and had no effect on the increase of A value of slices preconditioned with 2.0 MAC of sevoflurane. Conclusion The preconditioning of enflurane, isoflurane, and sevoflurane for 30 min can protect the rat cerebral cortical slices against 10 min OGD injury. Activation of the GABA A receptor may be contributed to the neuroprotective effects of enflurane and isoflurane.
出处 《上海第二医科大学学报》 CSCD 北大核心 2005年第5期511-514,共4页 Acta Universitatis Medicinalis Secondae Shanghai
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