深冻异体骨复合自体骨髓修复兔桡骨节段性骨缺损

Repairing radius segmental bone defects of rabbits with deep-low-temperature frozen allogeneic bone combined with autologous bone marrow

目的:研究兔深冻异体骨复合自体骨髓对节段性骨缺损的修复及其机制。方法:36只新西兰大白兔采用桡骨15mm节段性骨缺损模型,随机分为3组,A组植入深冻异体骨复合自体骨髓,B组植入深冻异体骨,C组植入自体骨。术后不同阶段分别行大体、组织学及X线检测。结果:(1)3组骨缺损均有成骨现象发生;(2)A、C组骨生成、骨连接情况明显优于B组;(3)A、C组骨缺损愈合时间较B组明显缩短,A、C组骨缺损均于术后8周愈合,B组骨缺损在术后10周仍未愈合。结论:提示兔深冻异体骨复合自体骨髓具有良好的组织相容性及成骨作用,其取代自体骨植骨修复骨缺损具有可能性。

Objective: To study repairing segmental bone defects with deep-low-temperature-frozen allogeneic bone combined with autologous bone marrow of rabbits and its mechanism. Methods: The 15mm segmental bone defects were created on bilateral radius in 36 New Zealand rabbits and were divided into three groups. Group A received deep-low-temperature-frozen allogeneic bone combined with autologous bone marrow, group B: deep-low-temperature-frozen allogeneic bone, group C: autogenous bone. General observation, HE histologic section,serum Alkaline phosphatase, transmission electron microscope and Xray were done in different stages. Results: (1) Three groups were all repaired. (2) Bone formation and synostosis of group A and C surpassed group B obviously. (3) The bone defects healed in the 8th week in group A and C, but did not healed in the 10th week in group B. Conclusion: The deep-low-temperature-frozen allogeneic bone combined with autologous bone marrow of rabbits has good histocompatibility and osteogenesis;It's possible to repairing bone defects with deep-low-temperature-frozen allogeneic bone combined with autologous bone marrow displacing autogenous bone .