摘要
圆叶决明在0℃低温下分别胁迫6、12、18、24h后,叶片叶绿素总量较对照(25℃)下降21.89%、26.04%、28.16%、29.31%,可溶性蛋白质含量、H2O2含量和·O-2净产生速率随胁迫时间增加,活性氧清除酶SOD的活性随胁迫时间先上升后下降。施Ca2+处理可明显降低圆叶决明叶片·O-2净产生速率、H2O2含量和细胞质膜的电解质渗透率,同时提高了叶绿素含量和抑制活性氧的产生,其中以0.5mmol/L浓度的CaCl2处理效果为最佳,说明Ca2+对低温胁迫下圆叶决明的膜结构起有效的保护作用。
After 6, 12, 18 and 24 hours' treatment with 0°C chilling stress on Chamaecrista rotundifolia, the contents of chlorophyll (chl) decreased by 21.89%, 26.04%, 28.16% and 29.31% than CK(25°C), respectively, dissolubility protein content, H2O2 content and ·O2- net generation rate increased with chilling stress time, the scavenging enzymes (SOD) activity increased at earlier stage and then dropped. The treatment of Ca2+ fertilizer under chilling stress could significantly decrease the net rate of ·O2- generation, the content of H2O2 and the leakage rate of electrolyte, the contents of scavenging enzymes SOD, meanwhile, the chlorophyll (chl) content increased. The effect of 0.5 mmol/L CaCl2 treatment was the best. The results indicated that Ca2+ could protect the membrane structure of Chamaecrista rotundifolia under chilling stress.
出处
《农业工程学报》
EI
CAS
CSCD
北大核心
2005年第6期110-113,共4页
Transactions of the Chinese Society of Agricultural Engineering
基金
福建省科技厅重点资助项目(2003N044)
关键词
低温胁迫
圆叶决明
钙肥
叶绿素
活性氧
过氧化物歧化酶
电解质渗透率
chilling stress
Chamaecrista rotundifolia
calcium fertilizer
chlorophyll (chl)
active oxygen
(superoxide) dismutase
leakage rate of electrolyte