正常T细胞分泌激活因子基因多态性与HIV-1感染者病毒载量、CD_4^+细胞计数和CD_4^+/CD_8^+比值的关系

Relationship of chemokine RANTES gene polymorphisms with plasma viral load,CD_4^+T cell and CD_4^+/CD_8^+ ratio in HIV-1 infected subjects

目的分析正常T细胞分泌激活因子(RANTES)启动子和内含子等位基因,对艾滋病病毒1型(HIV1)感染者外周血CD+4细胞计数、CD+4/CD+8比值和病毒载量的关系,以期探讨RANTES单核苷酸多态性(SNP)和艾滋病(AIDS)发病的关系。方法用流式细胞仪和realtime法对40例汉族HIV1感染者测定外周血CD+4、CD+8细胞计数和病毒载量,应用PCRRFLP法进行基因分型。结果对RANTES403、28和In1.1三个位点等位基因与外周血CD+4T淋巴细胞计数、CD+4/CD+8比值和病毒载量的关系的分析表明,具有RANTESIn1.1C/C和T/C基因型的感染者,与较高的病毒载量、较低的CD+4T细胞计数和CD+4/CD+8比值有关。In1.1T/T与T/C、C/C基因型的log10病毒载量的差异有非常显著的统计学意义(P<0.01),但是不同基因型之间CD+4T淋巴细胞计数和CD+4/CD+8细胞比值则无显著性差异。log10病毒载量和CD+4T淋巴细胞计数(r=-0.447,P<0.01)、CD+4/CD+8比值(r=-0.369,P<0.05)有显著的负相关关系。结论所研究人群中,具有RANTESIn1.1C的基因型者,与较高的病毒载量、较低的CD+4T细胞计数和CD+4/CD+8比值有关,它们是反映AIDS进程的主要指标,可能加速AIDS发病进程;而RANTES403和28等位基因的影响则处于相对次要的作用。

Objective To analyze relationship of RANTES allele frequencies with plasma viral load,CD+4 cell count and CD+4/CD+8 ratio as indicators of AIDS progression.Methods Forty cases of HIV-1 infected blood donors of Han population from Henan province were investigated.PCR restriction fragment length polymorphisms(PCR/RFLP) were used for genotyping.Plasma HIV RNA levels were quantified using the HIV-1 RNA PCR fluorescence.CD+4,CD+8T cell count was determined by flow cytometry.Results Individuals with bearing In1.1T/C and RANTES-In1.1C/C genotypes had higher viral loads and lower CD+4T cell counts and CD+4/CD+8 ratio than those without bearing.Significant differences for log 10 viral loads were observed for RANTESIn1.1T/T with T/C,C/C.No significant correlation was found for CD+4 cell count and CD+4/CD+8 ratio.Significanty negative correlation between log 10viral load and CD+4 cell counts (r=-0.447,P<0.01) or CD+4/CD+8 ratio(r=-0.369,P<0.05) was observed.Conclusion RANTES In1.1T/C and C/C genotypes were associated with higher viral loads and lower CD+4T cell counts and CD+4/CD+8 ratio which affect AIDS progression.Individuals with In1.1C might increase susceptibility to AIDS.The other RANTES-403,-28 alleles were also associated with the gene susceptibility to disease progression in the Han Chinese population,but with less importance.