摘要
为获得最佳产酶配方,同时获得高活力的β-甘露聚糖酶,经碳源、氮源、碳氮质量比、初始pH和培养温度5个单因素发酵条件的优化,得到芽孢杆菌WY45发酵产β甘露聚糖酶的最适发酵培养条件:以4g/L魔芋精粉为碳源,1.33g/L大豆蛋白胨为氮源,碳氮质量比为4/1,初始pH5.5,50℃培养时间96h。此条件下,β甘露聚糖酶活性最高可达2800U/mL。
Bacillus subtilis WY45 for high activity of extracellular β-mannanase was isolated from soil samples. The fermentation conditions, including carbon source, nitrogen source, ratio of the two sources, cultivation temperature and pH value, were optimized in order to improve the activity and amount of the enzyme. The result showed that Bacillus subtilis WY45 grew best when cultured at 50 ℃ with the medium composed of 4% konjac powder as carbon source, 1.33% soya peptone as nitrogen source, and pH value 5.5. After 96 h culturing, the enzyme show the highest activity of 2800 U/mL that is the highest value obtained up to now.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2005年第3期77-80,共4页
Journal of China Agricultural University
基金
国家"十五"科技攻关计划项目(2004BA713C04-02)
关键词
芽孢杆菌
Β-甘露聚糖酶
发酵
魔芋粉
Bacillus subtilis
β-mannanase
fermentation
konjac powder
