4种抗菌药物对幽门螺旋菌体外抗菌活性的测定

ANTIBACTERIAL ACTIVITY OF FOUR ANTIBACTERIAL AGENTS ON THE HILICOBACTER PYLORI IN VITRO

本文采用琼脂倍比稀释法测定了４种抗菌药物对幽门螺旋菌（ＨｅｌｉｃｏｂａｃｔｅｒＰｙｌｏｒｉ，ＨＰ）的ＭＩＣ。枸橼酸秘对４２株ＨＰ的ＭＩＣ范围为５－１００μｇ／ｍｌ，ＭＩＣ＿（５０）为２０μｇ／ｍｌ，ＭＩＣ＿（９０）为５０μｇ／ｍｌ；氨苄青霉素和先锋霉素Ｖ对３２株ＨＰ的ＭＩＣ范围分别为０．００３９－０．１２５μｇ／ｍｌ及０．００２０－０．２５μｇ／ｍｌ，ＭＩＣ＿（５０）分别为０．０１５６μｇ／ｍｌ及０．０３１３μｇ／ｍｌ，ＭＩＣ＿（９０）为０．０６２５μｇ／ｍｌ；氟哌酸对２６株ＨＰ的ＭＩＣ范围为０．５－１６μｇ／ｍｌ，ＭＩＣ＿（５０）为２μｇ／ｍｌ，ＭＩＣ＿（９０）为１６μｇ／ｍｌ。枸橼酸铋对ＨＰ的ＭＩＣ范围较大，５％菌株ＭＩＣ为１００μｇ／ｍｌ。对其它三药ＭＩＣ范围较小，浓度较低，说明ＨＰ对以上药物均敏感，但对枸橼酸铋可能存在耐药菌株。

he minimum inhibitory concentration(MIC) of 4 antibacterial agents on helicobacter pylori(HP) was tested by the agr double dilution methods. Minimum inhibitory concentration (MIC),MIC50 and MIC90 were used to evaluate their antibacterial activity.The results showed that MIC,MIC50 and MIC90 were 5-100μg/ml,20μg/ml,and 50μg/ml for tripotassium dicitrate bi-smuthate(TDB) in 42 HP trains,0.004 - 0.125μg/ml,0.016μg/ml,and 0.063μg/ml, for ampicillin in 32 HPtrains, 0.002 - 0.25μg/ml,0.031μg/ml,and 0.063μg/ml,for Cefazolin in 32 HP trains,and 0.5-16μg/ml,2μg/ml,and 16μg/ml for norfloxacin in 26 HP trains,respectively.The MIC of TDB were higher thanthose of other three drugs. This experiment indicats that some antibacterial agents can inhibite HPeffectively,but the partial resistant HP trains to drugs may exist.