摘要
本文报道了利用乙睛－甲醇－磷酸流动相体系分析生物膜磷脂的高效液相色谱法。所用色谱柱为ＳｈｕｎＰＡＫＣＬＣ－ＳＩＬ柱（１５０×６ｍｍ，５ｕ），检测波长２０５ｎｍ。该法可在４５ｍｉｎ之内分析８种膜磷脂组分，并可在１２ｍｉｎ之内使４种主要的红细胞膜磷脂得到完全分离。该法的线性范围宽，色谱的重复性较好，是一种简便、快速、实用性强的生物膜磷脂分析方法。
novel high performance liquid chromatographic method for analysis of phospholipids inbiomembrane is deseribed. The separation was performed on a CLC-SIL column with the mobilephase system of acetonitril-methanol-85%phosphoric acid,using isocratic elution and UVdetection at 205nm. completed separation of eight constituente of membrane phospholipids wasachieved within 45 min,and four major constituents of phospholipids in erythrocyte ghostmembrane could be separated completely within 12 min.This method is rapid,reliable,producibleand practical for determination of biomembrane phospholipids.
出处
《重庆医科大学学报》
CAS
CSCD
1994年第2期105-108,共4页
Journal of Chongqing Medical University
基金
四川省教委资助
关键词
高效液相色谱
膜磷脂
磷脂
High
performance liquid chromatography
Phospholipids
