羽衣甘蓝花药培养技术体系的研究

Studies on the Technical System of AntherCulture of Brassica oleracea var. acephala

本实验以6份羽衣甘蓝为材料进行花药培养.细胞学观察表明,当花蕾长为2.5-3.5mm,花瓣与花药长度之比为1/2～4/5时,45-65%的小孢子发育处于单核晚期,是花药培养的最佳时期.花药培养结果可见基因型不同,胚状体的诱导率有极大的差异,最容易形成胚状体的材料是Y3,诱导效率达17.74%;胚状体的出现在花药接种后的15-30天之间.培养基的种类不同,培养效果也有差异,其中以Keller培养基效果最佳;培养基中有机成份加倍,则可以显著提高胚状体的诱导效率.培养基中蔗糖的浓度对胚状体的诱导率也有显著的影响.

Six cultivars of Brassica oleracea var. acephala were used in the study anther culture. The main results are as follows: The cytological observations of pollen showed that most of the pollen were in the late uninucleate stage when the ratio of petal to anther length was between 1/2~4/5 in Brassica oleracea var. acephala ,and the stage of late uninucleate was optimal for anther culture. The effects of genotype and cultural medium on embryoid induction were investigated in s Brassica oleracea var. acephala. The results showed it was optimal to induce the embryoid in Keller medium with double organic ingredients and 10% sucrose. The capacity of embryoid production varied significantly in different genotypes, and genotype Y3 was an ideal material for embryoid induction .