使用SSR和mtVNTR分子标记识别扬子鳄个体

Individual identification of Chinese alligator Alligator sinensis with SSR and mtVNTR molecular markers

扬子鳄是中国特有的濒危物种,为有效避免种群衰退,最大限度地保持该物种现有的遗传多样性,有必要对种群的个体进行个体识别研究,以便重建遗传谱系,指导现有繁育工作。应用SSR(Simplesequencere-peats)与mtVNTR(VariablenumbertandemrepeatsonmitochondrialDNA)两种分子标记对扬子鳄39个个体进行了个体识别分析,结果显示:8个SSR座位的累计个体识别率与累计父权排除率分别达0·9968、0·7697,mtVNTR的个体识别率为0·9146,联合SSR与mtVNTR两种分子标记的累计个体识别率理论值达0·9997,并在实际分析中将39个扬子鳄个体完全区分开,其区分能力较RAPD(RandomamplifiedpolymorphicDNA)、AFLP(Amplifiedfragmentlengthpolymorphism)及mtDNA控制区5′端序列分析等分子标记要高。此外,SSR和mtVN-TR还可对某些低频等位基因及其携带个体做有效的筛查,这对今后进行大量扬子鳄个体的分子标记识别和群体的遗传谱系建立等工作将具有一定实际意义[动物学报51(3):501-506,2005]。

The Chinese alligator is an endemic species in China. To prevent its genetic depression and protect genetic diversities in the current population, individual identification of alligators is an essential issue for the reconstruction of pedigrees of animals bred in captivity. Therefore, SSR and mtVNTR markers were adopted to discriminate 39 individual alligators sampled from a captive population and skeletons. The result showed that the cumulate power of discrimination (CDP) and cumulate probability of paternity exclusion (CPE) of 8 SSR loci were 0.9968 and 0.7697 respectively and the power of discrimination (DP) of mtVNTR was 0.9146. A combined analysis of 8 SSR loci and mtVNTR gave out the CDP value at 0.9997, showing a powerful ability in distinguishing individual alligator s. In the present study, all individuals are clearly labeled with SSR loci and mtVNTR molecular markers that will be useful in individual identification and pedigree construction.