Slp2基因的差异表达与肺癌的分化和转移

The Correlation between the Differential Expression of Slp2 Gene(stomatin like protein-2) and the Differentiation and Metastasis of the Lung Cancer

目的:探讨Slp2基因在肺癌组织中的差异表达与肺癌组织分化和转移的相关性。方法:通过提取52例肺癌患者的肺癌组织和正常配对组织以及4个肺癌细胞系的总RNA,用逆转录-聚合酶链反应检测Slp2基因的表达,结合肺癌患者的临床资料进行分析。结果:52例肺癌组织中38例Slp2基因的表达明显增强。其中低年龄、低分化、纵隔淋巴结转移、Ⅲa期肺癌患者Slp2基因的差异表达更为明显(P<0.05);Slp2基因在4个肺癌细胞系中均呈阳性表达。结论:Slp2基因在肺癌组织的表达明显高于正常对照组织,而且与肺癌组织的分化程度、是否发生纵隔淋巴结转移以及临床分期密切相关。

Objective: To study the correlationship between the Slp2 gene expression and the prognosis evaluation of lung cancer. Methods: Total RNAs were extracted from 52 patients with lung cancer, and in four lung cancer cell lines (A549, GLC-82, NCI-H446, NCI-H460), Slp2 gene expressions were detected at mRNA level by reverse transcription-polymerase chain reaction (RT-PCR). Results: Up-regulation of the Slp2 gene was shown in 29 of the 52 (63.0%) lung cancer patients and all of the lung cancer cell lines, especially in younger patients (89.5% vs 44.4%, P<0.01 ), poorly differentiated lung cancer patients (81.0% vs 48.0%, P<0.05), mediastinum lymphonodus metastasis (77.3% vs 20%, P<0.05) and the patients with Ⅲa-stage lung cancer (73.1% vs 0, P<0.05). Conclusions: The expression of Slp2 gene is significantly higher in the tissue of lung cancer than in the normal controls, and is closely correlated with differentiation of the lung cancer tissue and the fact whether or not the metastasis of mediastinal lymph node occurs, as well as clinical staging, etc.