分泌性中耳炎中耳积液中γ干扰素测定的临床意义

The significance of IFN-γ measurement in middle ear effusion of secretory otitis media

目的:探讨分泌性中耳炎(SOM)患者的中耳积液中IFNγ浓度测定的临床意义。方法:用双抗夹心酶联免疫吸附法检测51例(56耳)SOM患者(中耳炎组)的中耳积液、血清及24例正常成人(对照组)血清中IFNγ的浓度。结果:中耳炎组积液中IFNγ的浓度明显高于其血清中的浓度(P<0.01);中耳炎组与对照组血清中的浓度差异无统计学意义(P>0.05);SOM慢性期IFNγ浓度明显高于急性期(P<0.05);首次穿刺及第2次穿刺积液中IFNγ的浓度差异无统计学意义(P>0.05);而第3次或3次以上穿刺积液中IFNγ的浓度则明显升高(P<0.01)。结论:中耳积液中的IFNγ可由中耳腔局部产生,而非单纯由血液中渗透而来;中耳积液中IFNγ的高浓度可作为SOM转为慢性病程或迁延不愈的参考。

Objective:To explore the clinical significance of IFN-γ in secretory otitis media(SOM) by measuring and analyzing and the level of INF-γ in the effusion of middle ear.Method:A commercially available enzymelinked immunosorbent assay (ELISA) incorporating a biotinylated monoclonal antibody probe was utilized for the quantitative measurement of IFN-γ in the middle ear effusion and serum samples of the patients and that of the normal persons.Result:The level of IFN-γ in the middle ear effusion was much higher than that in the serum samples.The difference of the level of IFN-γ in the serum samples is not significant between the test group and the control group.The level of IFN-γ in chronic period was much higer that in acute period.The level of IFN-γ in the middle ear effusion has no significant difference between the group undergoing tympanocentesis for the first time and the group undergoing tympanocentesis for the second time(P>0.05).The levels of IFN-γ in the middle ear effusion in the group undergoing tympanocentesis for three or more times arose obviously (P<0.01).Conclusion:The IFN-γ in the middle ear effusion may be produced by local tympanum,but not exuded simply from blood.The high expression of IFN-γ in the middle ear effusion may be a reference of SOM tending to chronic coures.