小麦转化受体体系的建立——小麦幼胚组织培养研究

The Establishment of a Transformed Receptor System in Wheat——Tissue Culture of Wheat Embryos

建立了一个以小麦幼胚为转化受体,转化后快速成苗的体系。筛选出适合于不同小麦品种的愈伤组织诱导培养基:MS+2mg·L-12,4-D+0.4mg·L-1IAA+0.2mg·L-1KT,愈伤诱导率达94.87%～99.04%。幼胚接种后,暗处理与弱光、强光合理配置利于绿芽的快速形成,在分化培养基中添加2.0g·L-1活性炭或1mg·L-1多效唑利于小麦再生苗的正常生长,从而提高移栽成活率。

A transformed receptor system of wheat, which used young embryos as receptor and speeded up regeneration and formation of plantlets for the transformed embryos, was established by improving culture conditions of immature embryos. The culture medium, MS+2mg·L-1 2,4-D+0.4mg·L-1 IAA+0.2mg·L-1 KT had a better effect on callus induction of different spring wheat immature embryos, the induction rate could come up to 94.87%～99.04%. The newly produced callus, exposed under weak light, was easy to grow into green buds and regenerate. 2.0g·L-1 active charcoal or 1mg·L-1 Paclobutrazol could promote the regeneration of green buds, strength of seedling and development of root. The autoclaved nutrient soil had good effect on the growth of the cultured wheat seedlings when they were transplanted.