地被菊幼嫩花瓣组织培养研究

Studies on Tissue Culture of Ground-cover chrysanthemum group′s Flowering Organ

以地被菊幼嫩花瓣为试材,进行了组织培养的研究,结果表明地被菊幼嫩花瓣是诱导愈伤组织的良好材料。当培养基中2.4-D和NAA浓度为0.5～2mg·L-1时,愈伤组织诱导率均达87.2%以上,并且诱导出的愈伤组织结构疏松,易于分割。在分化培养基MS+KT2mg·L-1+IAA0.5mg·L-1上,芽分化率可达92.5%。不同接种方向对地被菊花瓣不定芽形成无显著影响。地被菊花瓣培养芽的分化率以基部组织为最高(94%),中部和上部组织分化率则逐渐变低。经培养得到的再生植株与原品种相比在形态上产生了一系列变化。

Studies have been conducted on the tissue culture of Ground-cover Chrysanthemum Group's young petal. The result indicate that the young petal of ground-cover chrysanthemum group is the best for callus induction and plant regeneration. The best callus induction frequency(87.2%)was obtained in the medium of BA and 2.4-D0.5～2㎎·L-1. After the callus was transferred to the regeneration medium(MS+KT2mg·L-1+IAA0.5mg·L-1), the bud regeneration frequency reached 92.5%.The percentage of bud differentiation of base tissue(94%)is significantly better than that of middle and upper tissue of petal. Different directions of inoculation had no significant effect on the adventitious bud formation in petal culture of ground-cover chrysanthemum group, but significant differences appeared among the cultivars. The regeneration plants produced a series of changes compared with the original variety.