中药“参阳”方调节舌鳞癌SD大鼠血清Th1/Th2型细胞因子的实验研究

Modulation of Chinese prescription Shenyang on Th1/Th2 cytokines in serum of SD rats with squamous cell carcinoma of the tongue

目的:观察“参阳”方对舌鳞癌SD大鼠血清Th1/Th2型细胞因子表达水平的影响,并探讨其可能的作用机制。方法:80只SD大鼠采用0.002%4-硝基喹啉-1-氧化物自然饮水喂养36周,诱发建立舌鳞癌动物模型61只,随机分为4组:“参阳”方A组、B组、阳性对照(刺五加)组和阴性对照组。药物干预15d,于服药前后制备外周静脉血血清,酶联免疫吸附法测定Th1型细胞因子IFN-γ、IL-2、TNF-α和Th2型细胞因子IL-4、IL-10的含量,自身前后对照,配对t检验进行统计学分析。结果:“参阳”方可显著增加血清中Th1型细胞因子TNF-α和IL-2的含量(P<0.05),对血清中IFN-γ的含量也有一定的增加作用,同时能显著降低Th2型细胞因子IL-4和IL-10的表达(P<0.05)。结论:逆转Th1向Th2漂移,从而增强机体的细胞免疫功能,可能是“参阳”方发挥抗癌作用的主要效应机制之一。

PURPOSE:Prospective research demonstrated that Chinese prescription Shenyang could prolong the survival time and improve the survival rate of patients with oral squamous cell carcinoma (SCC). But the mechanism was not clear. The purpose of this study was to investigate effect of Shenyang on the level of Th1/Th2 cytokines in serum of SD rats with SCC of tongue and clarify its mechanism. METHODS: Among 80 SD rats fed by 0.002% 4-nitroquinoline-1-oxide (4NQO) drinking water for 36 weeks, 61 with SCC of tongue had been found and randomly divided into 4 groups, namely Shenyang A, Shenyang B, positive and negative control group. Before and after high and low dosage of Shenyang , acanthopanax senticoside and water had been given for 15 days respectively, peripheral blood serum was collected. Secretion of Th1-type cytokines, such as IFN-γ, IL-2, TNF-α and Th2-type cytokines, such as IL-4, IL-10 were detected by enzyme-linked immunosorbent assay (ELISA). Statistical analysis was made with paired t test. RESULTS: Shenyang significantly enhanced ,the serum levels of Th1-type cytokine, TNF-α and IL-2 (P<0.05), and decreased the expression of Th2-type cytokine, IL-4 and IL-10(P<0.05). Serum IFN-γ had been increased to some extent. CONCLUSION: Reversing Th2 to Th1 cytokines and then enhancing the immunity may be one of the mechanisms in anti-tumor effect of Shenyang. Supported by Science and Technology Development Fund (No.014319359) of Shanghai Municipality.