急性一氧化碳中毒大鼠血小板活性的变化及其高压氧暴露的影响

Changes of activity of thrombocyte in mice after acute carbon monoxide poisoning and the effect of hyperbaric oxygen on them

目的观察急性一氧化碳中毒(ACOP)大鼠血小板活性的变化及其高压氧(HBO)暴露的影响。方法将实验动物Wistar大鼠共128只随机分成正常对照组(NC组)、一氧化碳中毒组(COP组)、一氧化碳中毒+高气压组(HPT组)及一氧化碳中毒+高压氧组(HBO组)。测定COP后1,5,10,15,20d大鼠血小板平均相对体积及血小板CD61的动态变化。结果COP后,血小板平均相对体积及血小板CD61的表达均显著升高(P<0.05),而且持续15d以上。HBO组较COP组血小板平均相对体积及血小板CD61的表达较快恢复正常(P<0.05)。HPT组与COP组相比高气压对血小板平均相对体积及血小板CD61的表达均无显著影响(P>0.05)。结论ACOP可导致血小板活性增加并持续。HBO治疗可以使其较快恢复正常。

Objective To observe changes of activity of thrombocyte in mice after acute carbon monoxide poisoning and the effect of hyperbaric oxygen on them. Methods 128 Wistar rats were randomly divided into four groups :control group, HPT groups, COP groups and HBO groups. Volume of thrombocyte and expression of thrombocyte CD^61 were measured by FCM( flow cytometer) on the 1,5,10,15,20 day after ACOP.Results (1) Volume of thrombocyte and expression of thrombocyte CD^61 both increased remarkably after ACOP and these changes lasted for at least 15 days(P<0.05). (2) These factors in the HPT group recovered more quickly than those of the COP group (P<0.05). (3) Compared with the COP group, hyperbaric air had no significant influence on ACOP rats (P>0.05) .Conclusions The activity of thrombocyte increased remarkably and continuously after ACOP ; HBO treatment can make them recover more quickly .