小儿法乐四联症心肌细胞C-myc基因表达的病理学研究

Clinicopathological research on expression of myocardium c-myc in the congenital tetralogy of Falloet

目的探讨C-myc基因在法乐四联症发生、发展中的作用。方法采用免疫组织化学二步法检测C-myc基因在法乐四联症患者心肌细胞中的表达。结果C-myc基因在该症患者心肌细胞中的阳性表达率为86%(17/20),阳性细胞数为(41.7±2.8)%;其中强阳性(+++)6例(35.3%);阳性(++)7例(41.2%);弱阳性(+)4例(23.3%);正常对照组心肌细胞的阳性表达率仅为11.1%(2/18),且均为弱阳性;阳性细胞数为(5.4±1.1)%,两组相比差异非常显著(P<0.01)。结论C-myc基因在法乐四联症发生、发展中具有重要作用,C-myc基因的激活和高表达促进了该症的形成和发展。

Objective To study the importance of expression of myocardium c-myc gene in the congenital tetralogy of fallot.Methods The expression of myocardium c-myc gene in the congenital tetralogy of Fallot was investigated by immunohistochemical methods.Results The positive of myocardium c-myc gene was 85%(17/20), the positive cells were (41.7±2.8)%; In the positive of myocardium c-myc gene of 17cases, the strong positive(+++)was in 6 cases( 35.3%), the positive(++) of myocardium c-myc gene was in 7 cases (41.2%), the dimpositive (+) was in 4 cases(23.5%). In the controls, the positive of myocardium c-myc gene was 11.1%(2/18) and the positive cells were (5.4±1.1)%. There was significant difference between the congenital tetralogy of Fallot and the control(P<0.01;P<0.001). Conclusion There is relationship between the expression of myocardium c-myc gene and the progress of the congenial tetralogy of Fallot.