肝癌、肝硬化中自然杀伤细胞的免疫组织化学研究

Immunohistochemical study of natural killer cells in hepatocellular carcinoma and cirrhosis

目的研究原发性肝细胞癌、肝硬化、正常肝组织中自然杀伤(naturekiller,NK)细胞数量、分布及其与患者预后的关系。方法原发性肝细胞癌60例,单纯性肝硬化62例,正常肝组织23例,以SP免疫组化法检测NK细胞。结果①癌中与癌旁组织的NK细胞计数明显高于肝硬化(P<0.01),癌中NK细胞计数高于正常肝组织(P<0.05)。②肝癌组织学分级与NK细胞数量无明显关系。③癌中NK细胞随着临床分期的发展有下降的趋势(P<0.05)。④15月内转移复发组癌中和癌旁的NK细胞计数均明显低于无转移复发组(P<0.01)。结论NK细胞计数可能是反映机体抗肿瘤免疫状态和判断患者预后的重要指标。

Objective:To investigate the number and distribution of natural killer (NK) cells and its significance in hepatocellular carcinoma (HCC), cirrhosis and normal liver tissues.Methods:NK cells were examined in the tissue samples from 60 cases of primary HCC,62 cases of cirrhosis and 23 cases of normal liver by immunohistochemical methods with streptavidin-horseradish peroxidase detective system.Results:①The numbers of NK cells in HCC and its surrounding tissues were significantly higher than that in cirrhosis (P<0.01); the number in HCC was higher than that in the normal tissues (P<0.05). ②There was no relationship between the number of NK cells in HCC and their histological grades. ③The number of NK cells in HCC decreased along with the patient's clinical TNM stage (P<0.05). ④The number of NK cells in HCC and its surrounding tissues in the group with metastasis in 15 months were significantly lower than that without metastasis (P<0.01).Conclusion:The number of NK cells may be an important marker to estimate the HCC patient's immune status to predict the prognosis of patients.