原发性肝癌组织中巨噬细胞与微血管的计数及其临床意义

Count of TAM and MV in tissues of primary hepatocarcinoma and its clinical significance

目的研究原发性肝癌(primaryhepatocarcinoma,PHC)组织中肿瘤相关巨噬细胞(tumorassociatedmacrophage,TAM)、微血管(microvessel,MV)的计数及其临床病理意义,并探讨二者之间的相互关系。方法应用ABC免疫组化法对47例PHC手术切除标本常规石蜡包埋切片分别检测MV和TAM并高倍镜下计数。结果47例PHC癌组织中MV与TAM计数均显著高于癌旁组织;MV(67.30±13.68)个/HPvs(37.20±10.58)个/HP(P<0.01);TAM(70.27±17.93)个/HPvs(44.15±9.10)个/HP(P<0.01)。TAM与MV在术前AFU(α岩藻糖)≤10μg/L病例的计数显著高于AFU>10μg/L病例计数;TAM(74.13±18.33)个/HPvs(61.15±13.54)个/HP(P<0.05);MV(70.41±13.03)个/HPvs(59.97±12.69)个/HP(P<0.05)。伴转移病例癌组织中的MV计数(73.50±13.77)个/HP显著高于无转移病例(64.10±12.68)个/HP(P<0.05);TAM与MV在PHC中的计数与PHC的其他临床病理特征均无明显关系;癌组织中TAM计数与MV计数呈密切正相关(r=0.686,P<0.01)。结论TAM与MV计数与PHC的发生、进展关系密切;MV计数高的PHC易于发生浸润和转移;TAM与PHC的血管生成可能有着密切的关系。

Objective:To study the count of the tumor-associated macrophage (TAM) and microvessel (MV) in PHC tissue and its clinicopathological significance, explore and discuss the correlation between them.Methods:MV and TAM in fourty-seven samples resected in PHC operation with routime parffin-embeded were measured and counted under high power microscope with the immunohistochemical method of avidin-biotin complex.Results:The count of MV and TAM in the cancer tissues was significantly higher than that in the peri-cancerous tissues (MV,67.30±13.68/HP vs 37.20±10.58/HP,P<0.01;TAM,70.27±17.93/HP vs 44.15±9.10/HP,P<0.01); the count of TAM and MV in the patients with pre-operative AFU ≤10 μg/L in serum was significantly higher than that AFU >10 μg/L (TAM,74.13±18.33/HP vs 61.15±13.54/HP,P<0.05; MV,70.41±13.03 vs 59.97±12.69,P<0.05),the count of MV in the patients with metastasis (73.50±13.77/HP) was significantly higher than that of non-metastasis (64.10±12.68/HP)(P<0.05),there were no correlations among the count of MV,TAM and the other clinicopathological features (including pathological type,differentiated degree,the value of AFP,with or without SHCSP,cirrhosis,cancer emboli,gross shapes,and maximal diameter of mass,etc);the count of TAM was significantly correlated with the count of MV (r=0.686,P<0.01).Conclusion:The count of TAM and MV may be closely related to the carcinogenesis and the progress of PHC; the count of MV may be associated with tumor invasion and metastasis; TAM and PHC may be closely related to the tumor angiogenesis.