体外受精与卵细胞内单精显微注射后受精和胚胎质量的比较

Comparison of fertilization and embryonic development between conventional insemination and ICSI treatment.

目的 评估 ICSI与 IVF后的卵子受精和胚胎发育，了解不明原因不孕的病人在同一治疗周期中，通过 ICSI受精的胚胎是否较 IVF更好。方法 将 30例不明原因不孕的病人同一周期的卵子随机分为两组，分别行 ICSI和 IVF授精，比较各组间卵子的受精和胚胎质量。结果 不明原因不孕中 IVF授精后发生受精障碍 3例，占 10％ (3/30)，而 ICSI授精后无受精障碍发生； ICSI授精组与 IVF授精组比较，卵子受精率无明显差异，而 a类胚胎率差异显著

Objective To evaluate whether or not the fertilization and embryonic development after intracytoplasmic sperm injection(ICSI) is better than those of conventional insemination in vitro fertilization－ embryo transfer (IVF－ ET)program in the same cycle of unexplained factor infertility.Methods Combining conventional insemination and ICSI were performcd on the sibling oocytes in the same cycle,were carried out 30 cycles from September 1999 to June 2000,the oocytes were randomly divided into two subgroups:subgroup B1 for IVF and subgroup B2 for ICSI.The fertilization rate and quality of embryo were compared.Results The fertilizaton failure occurred in 3(10％ )of 30 cycles after convertional inseminatiion but none after ICSI.The proportion of good embryo after ICSI was significantly higher than that after IVF(P