摘要
本实验采用固定比率为1:1的4h连续自身给药实验模型,药物是经典的具有较强精神依赖性的盐酸吗啡。在低于形成身体依赖性的阈下剂量,大白鼠经过一定的训练后能形成主动的觅药行为。改变每次响应注射剂量后,大白鼠均能通过调整踏板次数来控制药物的摄入量,组间t检验踏板次数有显著性差异,而日剂量无明显差异,说明精神依赖性已经形成。大白鼠自身给药实验形成稳定模型的周期短,且药物进入大鼠静脉时不延期、不打扰动物,给药的剂量准确,影响因素少,所得到的实验结果稳定可靠。
The experiment was carried out with a 4 - hour continuous intravenous self-administration of morphine under fixed ratio 1: 1 (FR=1). Through several days' training, the rats learned to press lever and drug - seeking behavior was established even under the sub-threshold dose of physical dependence. The rats could maintain self - administration by increasing the response rate (P<0. 05) if the dose was reduced, or decreasing the response rate if the dose was increased, (P<0. 05). However, the total daily dose remained constant. The advantages of the experiment are as follows: 1. the formation of the model needs less time; 2. drug gets into the vein of rats without delay; 3. the rats will not be disturbed; 4. the dose taken is precise; 5. the result is reliable.
