掺食法建立小鼠安定身体依赖性模型

PHYSICAL DEPENDENCE ON DIAZEPAM IN MICE BY DRUG-ADMIXED FOOD INGESTION

本文以掺食法递增安定剂量由0.25%、0.5%至0.75%,喂食56 d,形成小鼠身体依赖性模型,进食安定的剂量高达2000 mg·kg^(-1)·d^(-1),戒断后10 h出现一系列的戒断症状,在饮食减少同时伴有体重的下降,二者与未戒断组相比均有显著性差异,而饮水量在戒断后反有增加.戒断后电惊厥率明显上升,以戒断后12 b为最显著达70%.喂食安定7 d组,戒断时亦可见轻度戒断症状,但电惊厥率与对照组相比无显著性差异.

An experimental model of physical dependence on diazepam in mice was established by drug-admixed food (DAF) method. After 56 days of diazepam administration, the consumption of the drug reached as high as 2000 mg·kg^(-1)·d^(-1)at the end of medication period. 10 hours after withdrawal of diazepam, a series of abstinence syndromes occurred, the ingestion of food decreased concomitant with loss of body weight, which showed significant difference when compared with non-withdrawal group. Water intake increased after drug withdrawal. The electric convulsion rate increased significantly and reached the peak value (70%)at the time of 12 hours after drug withdrawal. Mice in the group of 7 days administration of diazepam showed weak abstinence syndromes after cessation of drug administration, and there was no significant difference in electric convulsion rate when compared with the control group.