家兔油酸中毒性肺水肿时血液流变性变化的动态观察

Dynamic Observation of Hemorheologic Changes Pulmonary Edema Induced by Oleic Acid in Rabbits

本文连续6 1/2小时观察了8只家兔油酸中毒性肺水肿的血液流变学变化。实验用新西兰白兔8只,1.5～2.5kg,耳缘静脉注入油酸0.1ml/kg,于注油酸前和注油酸后1/2h、1 1/2h、3 1/2h、6 1/2h分别采动脉血3ml。测量血液流变学指标的变化。发现注油酸后1 1/2h开始中切变率(46.0s^(-1))和高切变率(115.0s^(-1)和230.0s^(-1))的全血还粘度逐渐升高,与注油酸前相比有显著差异(P<0.05),至6 1/2h时升高更明显,与注油酸前相比有非常显著差异(P<0.01);同时,血浆粘度也逐渐升高,与注油酸前相比有显著差异(P<0.05)。此外,还测量到注油酸后血浆纤维蛋白原含量和全血血小板最大聚集率也呈逐渐升高趋势,但与注油酸前相比没有显著差异(P>0.05)。结果提示:家兔油酸中毒性肺水肿时血液呈高粘滞状态,这种血液流变学状态的改变对肺水肿的形成可能有一定的作用。

Hemorheoiogic changes of pulmonary edema induced by oleic acid in 8 rabbits were observed during 6 1/2 h experiment in this study. The model was injection of oleic acid 0. 1ml/kg into vein in the ear of rabbits weighing 1.5～2.5kg. Hemorheologic parameters were measured pior to and 1/2, 1 1/2, 3 1/2, 6 1/2 h after injection. The whole blood reduced viscosity (WBRV) at middle (46.0s^(-l)) and high (115.0s^(-1), 230.0s^(-1)) stress rate was found to have a marked increase at 1 1/2 h after the injection (P<0.05) and reach maximum at 6 1/2 h (P<0.01). Simultaneously, the plasma viscosity also increased significantly (P<0.05). Besides, the concentraction of the fibrinogen in plasma and the whole blood platelet maximum aggregation also raised slow after the injection, but the difference was not significant(P>0. 05). The results showed that the blood of pulmonary edema induced by oleic acid was hyperviscosity, which may related to the pathogenesis of pulmonary edema.