抗HCG-B单克隆抗体酶免疫法进行血痕中妊娠鉴定

Identification of Pregnacy Bloodstains by ELISA Using Monoclonal Antibodies to the β-subunit of HCG

血痕妊娠鉴定在某些犯罪侦察案中有其重要的法医学鉴定价值。从血痕等检材中进行法医学妊娠鉴定已有多种方法报道。目前常用的HCG测定方法主要有乳胶凝集抑制试验(LAIT)，反向被动皿凝试验(LAT)以及一些LAT，LAIT的改良法。

Specific and hignly sensitive enzyme-linked immunosorbent assay(ELISA) was applied to identification of pregnacy bloodstains using monoclonal antibodies against the carboxylterminal portion of β-subunit. When experimentally prepared bloodstains(about 30 μl blood)were submitted to analysis, OD values in Pregnaoy Bloodstains is 0.39+0.16; OD value in nopregnacy bloodstains is 0.03±0.06. The reaction did not chang significantly when these bloodstains were stored at room temperture for three months. After three months HCG confers in pregnacy bloodstains decreased with increacing aged bloodstains. The HCG cotents can be detected in pregnacy bloodstains exposed to the sun for two months.