溶菌酶、过氧化氢对壳聚糖降解性能的影响

Depolymerization of Chitosan with the Aid of Lysozyme and Hydrogen Peroxide

以溶菌酶和过氧化氢为催化剂研究了壳聚糖降解速率的变化。研究结果表明:溶菌酶可以大大提高壳聚糖的降解速率。无溶菌酶时,壳聚糖11天降解了19%;而在溶菌酶的作用下,壳聚糖11天降解了44%。若将溶菌酶与壳聚糖复合在一起形成缓释材料后,壳聚糖降解速率要比直接在溶菌酶溶液中的降解速率慢,前者29天降解了38%,后者29天降解了53%。在利用过氧化氢作为催化剂研究壳聚糖的降解行为时发现:当过氧化氢的浓度小于0.03×10-5g mL时,过氧化氢对壳聚糖的降解基本无效果,而高于此浓度时,过氧化氢则大大提高了壳聚糖的降解速率。选择不同过氧化氢浓度便可有效调节壳聚糖的降解速率。

The degradation rate of chitosan with lysozyme and hydrogen peroxide as catalysts was investigated. It was found that lysozyme can appreciably accelerate the degradation of chitosan. 44% of chitosan can be degradated with lysozyme in 11 days, but only 19% of chitosan is degradated without lysozyme. When chitosan is packed with lysozyme, its degradating rate is slower than that in lysozyme solution. The former rate is 38% in 29 days, and the later is 53% in 29 days. When hydrogen peroxide is used as catalyst, concentration should be no less than 0.03×10^(-5) g/mL, because the degradation of chitosan is accelerated only when the concentration of H_2O_2 is higher than 0.03×10^(-5) g/mL. Varying the concentration of H_2O_2 can control the degradation rate of chitosan.