复方银杏胶囊镇痛作用及其机制的实验研究

Studies on Analgesic Effect and Mechanism of Compound Ginkgo Capsule

目的:研究复方银杏胶囊的镇痛作用及其机制。方法:大鼠电刺激法测定痛阈;用试剂盒比色法测定脑组织中谷氨酸的含量;原子吸收光谱法测定脑组织中钙离子(Ca2+)浓度;免疫组织化学ABC法观察脑组织中N-甲基-D-天门冬氨酸I型受体(NMDAR1)的分布。结果:复方银杏胶囊350mg/kg可显著提高大鼠电刺激嘶叫阈(P<0.05),700mg/kg显著减少小鼠脑组织中谷氨酸的释放以及脑内钙离子浓度(P<0.05);复方银杏胶囊700mg/kg、350mg/kg可明显减少疼痛模型小鼠大脑皮层NMDAR1阳性细胞数(P<0.01,P<0.05)。结论:复方银杏胶囊具有良好的镇痛效应,其镇痛作用可能与拮抗NMDA受体从而抑制脑组织中谷氨酸的释放及钙离子内流有关。

Object To study the analgesic effect and mechanism of Compound Ginkgo Agent (ginkgo,Panax notoginseng,Ligusticum Chuanxiong Hort,etc.).Methods The analgesic effect of Compound Ginkgo Capsule(CGC) was assessed by the electrical stimulation in rats.The content of glutamic acid in the brain of analgesic model mice was assessed after taking CGC.The concentration of Ca 2+ was assessed by Atom Absorption Spectrometry(AAS).The distribution of NMDA receptor in the brain was assessed by Immunohistochemical method.Results Taking high dose of CGC( 350 mg/kg ig) after 1.0 h could significantly increase pain threshold in the electrical stimulation test(P<0.05).Taking high dose of CGC( 700 mg/kg ,ig) could significantly decrease the content of glutamic acid and the concentration of Ca 2+ in the brain of analgesic model mice(P<0.05).Taking high and middle dose of CGC( 700 mg/kg , 350 mg/kg ig) could significantly decrease the concentration of NMDA receptor in the brain of analgesic model mice (P<0.01,P<0.05).Conclusion CGC shows significant analgesic effect and its effect was acted by inhibiting NMDA receptor and decreasing the release of glutamic acid then inhibiting Ca 2+ .