中华芦荟多糖对辐射后非瘤细胞周期改变及周期相关蛋白表达的影响

The Modulating of Aloe Polysaccharides on the Cell Cycle and Cycle Regulating Protein Expression in X-ray Irradiated Non-malignant Cells

目的研究中华芦荟多糖(AP)对X射线照射后人非瘤细胞株C.Liver和293细胞周期进程及细胞周期相关蛋白表达的影响。方法流式细胞仪检测细胞周期,Westernblot检测细胞周期相关蛋白的表达。结果辐射后293和C.Liver细胞均出现明显的G2/M期阻滞,G0/G1期细胞比例显著下降,50μg/mlAP能显著改变上述趋势。AP预处理能显著下调辐射所致C.Liver细胞P53蛋白水平,显著升高P21、CyclinB1、pRb蛋白的表达,对P27、CDK4和CyclinD1蛋白表达水平无显著影响。结论AP对X射线辐射后非瘤细胞周期紊乱的改善作用与对周期相关蛋白表达的调节有关。

Objective:To study the modulating of Aloe Polysaccharides on the cell cycle and cycle regulating protein expression in X-ray irradiated non-malignant cells.Methods:The cell cycle was analyzed by flow cytometric analyzed.The levels of cell cycle regulating protein expression were tested by Western blot.Results:A distinct G_2/M block happened in 293 and C.Liver cells after irradiation.The pre-treatment of AP in the concentration of 50 μg/ml caused an increasing G_0/G_1 phase population and decreasing G_2/M phase population.Meanwhile,pre-treatment of AP could significantly decrease the high expression of p53 protein caused by irradiation and evidently enhance the expression of P21,Cyclin B1 and pRb protein.Pre-treatment of AP had no evident effect on p27,CDK4 and Cyclin D_1 protein.Conclusion:There is a radioprotective effect of AP on non-malignant cells.This effect is related to alleviating the cell cycle turbulence.The modulating of Aloe Polysaccharides on the cell cycle regulating protein expression in X-ray irradiated non-malignant cells contributes to its alleviating effect on the cell cycle turbulence.